FairBiotech's Tissue Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from tissue sample. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. The specialized high-salt buffer system allows the bases of RNA species to bind to the the glass fiber matrix of the spin column. Contaminants pass through the column easily and impurities are efficiently washed away. Purified RNA is eluted with REL Buffer without requirement of phenol extraction or alcohol precipitation. RNA purified with The Total RNA Isolation Kit is suitable for a variety of routine applications including RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNA Selection. The entire procedure can be completed within 25-40 minutes.